Results
| tube name | Hyper Ladder 1kb | GG DONR | pENTR Lyn-mKate 1 | pENTR Lyn-mKate 1 | pENTR Lyn-mKate 2 | pENTR Lyn-mKate 3 | pENTR Lyn-mKate 4 | pENTR Lyn-TEV 1 | pENTR Lyn-TEV 1 | pENTR Lyn-TEV 2 | pENTR Lyn-TEV 3 | pENTR Lyn-TEV 4 | pENTR Lyn Stop 1 | pENTR Lyn Stop 1 | pENTR Lyn Stop 2 | pENTR Lyn Stop 3 | pENTR Lyn Stop 4 | Hyper Ladder 1 | Hyper Ladder 1 | GG DONR | pENTR Syk-eYFP 1 | pENTR Syk-eYFP 1 | pENTR Syk-eYFP 2 | pENTR Syk-eYFP 3 | pENTR Syk-eYFP 4 | ggDONR | pENTR CD79B Gal4VP16 1 | pENTR CD79B Gal4VP16 1 | pENTR CD79B Gal4VP16 2 | pENTR CD79B Gal4VP16 3 | pENTR CD79B Gal4VP16 4 | pENTR Gmab Heavy 1 | pENTR Gmab Heavy 1 | pENTR Gmab Heavy 2 | pENTR Gmab Heavy 3 | pENTR Gmab Heavy 4 | Hyper Ladder 1 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 |
| Enzyme Name | PvuII | undigested | PvuII | PvuII | PvuII | PvuII | undigested | PvuII | PvuII | PvuII | PvuII | undigested | PvuII | PvuII | PvuII | PvuII | NsiI | undigested control | NsiI | NsiI | NsiI | NsiI | PciI | undigested | PciI | PciI | PciI | PciI | undigested | PciI | PciI | PciI | PciI | ||||
| result | F | P? | F | F | F | F | F | P? | P | P | P | P | P | P | P | P | |||||||||||||||||||||
| notes | looks like there are two bands really close together, which there should be | middle band seems slightly shorter than it should be |
| tube name | Hyper Ladder 1 | ggDONR | pENTR CD79B stop 1 | pENTR CD79B stop 1 | pENTR CD79B stop 2 | pENTR CD79B stop 3 | pENTR CD79B stop 4 | pZDoner 3-GTW-4 | pEXPR 3-hef1a:CD79A-4 1 | pEXPR 3-hef1a:CD79A-4 1 | pEXPR 3-hef1a:CD79A-4 2 | pEXPR 3-hef1a:CD79A-4 3 | pEXPR 3-hef1a:CD79A-4 4 | pEXPR 3-hef1a:CD79A -tcs-gal4vp16-4 1 | pEXPR 3-hef1a:CD79A -tcs-gal4vp16-4 1 | pEXPR 3-hef1a:CD79A -tcs-gal4vp16-4 2 | pEXPR 3-hef1a:CD79A -tcs-gal4vp16-4 3 | pEXPR 3-hef1a:CD79A -tcs-gal4vp16-4 4 | Hyper Ladder 1 | Hyper Ladder 1 | ggDONR | pENTR Gmab Light 1 | pENTR Gmab Light 1 | pENTR Gmab Light 2 | pENTR Gmab Light 3 | pENTR Gmab Light 4 | pZDonner 2-GTW-3 | pEXPR 2-TRE:Syk-TEV-3 1 | pEXPR 2-TRE:Syk-TEV-3 1 | pEXPR 2-TRE:Syk-TEV-3 2 | pEXPR 2-TRE:Syk-TEV-3 3 | pEXPR 2-TRE:Syk-TEV-3 4 | pEXPR 2-TRE:Syk-3 1 | pEXPR 2-TRE:Syk-3 1 | pEXPR 2-TRE:Syk-3 2 | pEXPR 2-TRE:Syk-3 3 | pEXPR 2-TRE:Syk-3 4 | Hyper Ladder 1 |
| gel/lane | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 |
| Enzyme Name | PciI/KpnI | PciI/KpnI | PciI/KpnI | PciI/KpnI | PciI/KpnI | PciI/KpnI | KpnI | undigested | KpnI | KpnI | KpnI | KpnI | undigested | KpnI | KpnI | KpnI | KpnI | NsiI | undigested | NsiI | NsiI | NsiI | NsiI | BamHI | undigested | BamHI | BamHI | BamHI | BamHI | undigested | BamHI | BamHI | BamHI | BamHI | ||||
| result | F | P? | P? | P? | P | P | P | P | P | P | P | P | P | F | P | P | P | P | P | P | P | P | F | P | ||||||||||||||
| notes | doesn't look like control, but there should be a band around 200 |
Virtual gels from Geneious
| pENTR CD79B stop | pENTR CD79B Gal4VP16 | pENTR Gmab Heavy | pENTR Gmab Light | pEXPR 3-hef1a:CD79A -4 | pEXPR 3-hef1a:CD79A-tcs-gal4vp16 -4 | pEXPR 2-TRE:Syk-TEV-3 | pEXPR 2-TRE:Syk-3 | Fail state (ggDONR) | Fail state (ggDONR QQ ligation) | fail pZDoner 3-GTW-4 | fail pZDonner 2-GTW-3 | ||||
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