Results
| tube name | Hyper Ladder 1kb | pEXPR cd79B-tcs-gal4vp16 1 | pEXPR cd79B-tcs-gal4vp16 2 | pENTR syk-eyfp 11 | pENTR syk-eyfp 12 | pENTR syk-eyfp 13 | pENTR syk-eyfp 14 | pENTR syk-eyfp 15 | pENTR syk-eyfp 16 | pENTR syk-eyfp 17 | pENTR syk-eyfp 18 | pENTR syk-eyfp 19 | pENTR syk-eyfp 20 | pEXPR cd79B-tcs-gal4vp16 1 | pENTR syk-eyfp 11 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| DNA concentration (ng/ul) | 242.7 | 195 | 296.8 | 90.8 | 83.7 | 103.2 | 87.6 | 43.7 | 40.2 | 122.9 | 38.1 | 57.8 | |||
| Enzyme Name | ApaI | ApaI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | undigested | undigested | |
| Buffer Name | |||||||||||||||
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| DNA volume | #DIV/0! | 2.06 | 2.56 | 1.68 | 5.51 | 5.97 | 4.84 | 5.71 | 11.44 | 12.44 | 4.07 | 13.12 | 8.65 | #DIV/0! | #DIV/0! |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| water volume | #DIV/0! | 14.9 | 14.4 | 15.3 | 11.5 | 11.0 | 12.2 | 11.3 | 5.6 | 4.6 | 12.9 | 3.9 | 8.3 | #DIV/0! | #DIV/0! |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| results | |||||||||||||||
| notes | probably no dna actually got into the gel. |
Virtual gels from Geneious
Syk-eyfp | pEXPR hEF1a: CD79B+STOP | ggDONR | pZDonor 3 gtw 4 |
|---|---|---|---|
 |  |  |  |