Results
| tube name | Hyper Ladder 1kb | pZDonor 1-2 | pEXPR Lyn-TEVp 1 | pEXPR Lyn-TEVp 1 | pEXPR Lyn-TEVp 2 | pEXPR Lyn-TEVp 3 | pEXPR Lyn-TEVp 4 | pEXPR Lyn-TEVp 5 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| DNA concentration (ng/ul) | 377.9 | 153.4 | 153.4 | 31.6 | 71.5 | 531.2 | 370.3 | |
| Enzyme Name | BsaI | undigested | BsaI | BsaI | BsaI | BsaI | BsaI | |
| Buffer Name | ||||||||
| gel/lane | 1 | 2 | 3 | 3 | 5 | 4 | 7 | 5 |
| DNA volume | #DIV/0! | 1.32 | 3.26 | 3.26 | 15.82 | 6.99 | 0.94 | 1.35 |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| water volume | #DIV/0! | 15.7 | 13.7 | 13.7 | 1.2 | 10.0 | 16.1 | 15.6 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| results | F | F | F | F | F | F | ||
| notes | digested by accident |
Table
Virtual gels from Geneious
| pEXPR Lyn-TEVp | pZDonor 1-GTW-2 |
|---|---|
 |  |