Results

Passes: GmabL4, CD79A 1-4, CD79B 1-4

Brian suggests we redo these transformations at 30C because big plasmids tend to get scrambled at 37.

Lane content1: ladder2: A/B 1 undigested3: A/B 14: A/B 2 undigested5: A/B 26: A/B 3 undigested7: A/B 38: A/B 4 undigested9: A/B 410: A/B4 1 undigested11: A/B4 112: A/B4 2 undigested13: A/B4 214: A/B4 3 undigested15: A/B4 316: A/B4 4 undigested17: A/B4 418: A4/B 1 undigested19: A4/B 120: ladder
pass/fail  All of these A/B's may have a tiny band at 8,000 and one at 200 (which ought be at like 300)          same as A/B's, there looks like a band at 200 in the undigested as well, I think these all failed.      
Lane content21: ladder22: A4/B 2 undigested23: A4/B 224: A4/B 3 undigested25: A4/B 326: A4/B 4 undigested27: A4/B 428: A4/B4 1 undigested29: A4/B4 130: A4/B4 2 undigested31: A4/B4 232: A4/B4 3 undigested33: A4/B4 334: A4/B4 4 undigested35: A4/B4 436: pDEST 4-537: Gmab L undigested38: Gmab L 139: Gmab L 240: ladder
pass/fail        maybe, the third "band" is 2 bands, and there should be one near 200, but I understand that maybe, the third "band" is 2 bands, and there should be one near 200, but I understand that maybe, the third "band" is 2 bands, and there should be one near 200, but I understand that       
Lane content41: ladder42: Gmab L 343: Gmab L 444: pDEST 1-245: CD79A undigested46: CD79A 147: CD79A 248: CD79A 349: CD79A 450: pDEST 2-351: CD79B undigested52: ladder        
pass/fail badGood  goodgoodgoodgood           
Lane content53: ladder54: CD79B 155: CD79B 256: CD79B 357: CD79B 458: ladder              
pass/fail goodgoodgoodgood               

 

 

 

Virtual gels from Geneious

 

A/BA/B4A4/BA4/B4TRE:GmabHTRE:GmabLTRE:CD79ATRE:CD79BAPPAPPSwe
  
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