Gels 2014-08-20

Created by Unknown User (eersland@mit.edu), last modified on Aug 21, 2014 13:55

Virtual gels from Geneious

tube name	Hyper Ladder 1kb	A/B 1	A/B 1	A/B 2	A/B 3	A/B 4	A/B 5	A4/B 1	A4/B 1	A4/B 2	A4/B 3	A4/B 4	A4/B 5	A/B4 1	A/B4 1	A/B4 2	A/B4 3	A/B4 4	A/B4 5	Hyper Ladder 1kb	Hyper Ladder 1kb	A4/B4 1	A4/B4 1	A4/B4 2	A4/B4 3	A4/B4 4	A4/B4 5	Hyper Ladder 1kb
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27
DNA mass desired (ng)	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500
total volume(ul)	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20
DNA concentration (ng/ul)		154.9	154.9	441.6	321.3	242.1	332.2	432.6	432.6	537.4	484.5	488.6	478.3	229.5	229.5	153.7	379.2	490.6	270.6			451.2	451.2	88	481.9	136.9	110.7
Enzyme Name		undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI			undigested	NcoI	NcoI	NcoI	NcoI	NcoI
Buffer Name
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27
DNA volume	#DIV/0!	3.23	3.23	1.13	1.56	2.07	1.51	1.16	1.16	0.93	1.03	1.02	1.05	2.18	2.18	3.25	1.32	1.02	1.85	#DIV/0!	#DIV/0!	1.11	1.11	5.68	1.04	3.65	4.52
buffer volume	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
enzyme volume	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
water volume	#DIV/0!	13.8	13.8	15.9	15.4	14.9	15.5	15.8	15.8	16.1	16.0	16.0	16.0	14.8	14.8	13.7	15.7	16.0	15.2	#DIV/0!	#DIV/0!	15.9	15.9	11.3	16.0	13.3	12.5
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27
loading dye	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4
results
notes

A/B	A/B4	A4/B	A4/B4

No labels