Gels 2014-08-21

tube name	Hyper Ladder 1kb	A/B 1	A/B 1	A/B 2	A/B 3	A/B 4	A/B 5	A4/B 1	A4/B 1	A4/B 2	A4/B 3	A4/B 4	A4/B 5	A/B4 1	A/B4 1	A/B4 2	A/B4 3	A/B4 4	A/B4 5	Hyper Ladder 1kb
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
DNA mass desired (ng)	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500
total volume(ul)	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20
DNA concentration (ng/ul)		154.9	154.9	441.6	321.3	242.1	332.2	432.6	432.6	537.4	484.5	488.6	478.3	229.5	229.5	153.7	379.2	490.6	270.6
Enzyme Name		undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI
Buffer Name		3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
DNA volume	#DIV/0!	3.23	3.23	1.13	1.56	2.07	1.51	1.16	1.16	0.93	1.03	1.02	1.05	2.18	2.18	3.25	1.32	1.02	1.85	#DIV/0!
buffer volume	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
enzyme volume	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
strip tube #	1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	2/1	2/2	2/3	2/4	2/5	2/6	2/7	2/8
water volume	#DIV/0!	13.8	13.8	15.9	15.4	14.9	15.5	15.8	15.8	16.1	16.0	16.0	16.0	14.8	14.8	13.7	15.7	16.0	15.2	#DIV/0!
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
loading dye	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4
results	all fail->													smear
notes		digested						digested				smear	These have fresh NsiI

Hyper Ladder 1kb	A4/B4 1	A4/B4 1	A4/B4 2	A4/B4 3	A4/B4 4	A4/B4 5	TRE:CD79A	TRE:CD79A	TRE:CD79B	TRE:CD79B	TRE:GmabL	TRE:GmabL	TRE:GmabH 1	TRE:GmabH 1	TRE:GmabH 2	TRE:GmabH 3	TRE Lyn-mKate	TRE Lyn-mKate	Hyper Ladder 1kb
21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40
500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500
20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20
	451.2	451.2	88	481.9	136.9	110.7	174.4	174.4	223	223	184	184	131	131	154.1	185.4	277	277
	undigested	NcoI	NcoI	NcoI	NcoI	NcoI	undigested	StuI	undigested	BamHI	undigested	BamHI	undigested	StuI	StuI	StuI	undigested	bglI
	3.1	3.1	3.1	3.1	3.1	3.1	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	3.1	3.1
21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40
#DIV/0!	1.11	1.11	5.68	1.04	3.65	4.52	2.87	2.87	2.24	2.24	2.72	2.72	3.82	3.82	3.24	2.70	1.81	1.81	#DIV/0!
2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
2/9	2/10	2/11	2/12	3/1	3/2	3/3	3/4	3/5	3/6	3/7	3/8	3/9	3/10	3/11	3/12	4/1	4/2	4/3	4/4
#DIV/0!	15.9	15.9	11.3	16.0	13.3	12.5	14.1	14.1	14.8	14.8	14.3	14.3	13.2	13.2	13.8	14.3	15.2	15.2	#DIV/0!
21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40
4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4
		good		good				could be good if extra band is undigested		good?, gels really wavy		good		probably good	probably good	probably good but not as good as the two over there <--		good
							looks digested							Poor enzyme choice, geneious had a wrong gel image first time

Hyper Ladder 1kb	hef1a:Lyn 2	hef1a:Lyn 2	hef1a:Lyn 3	hef1a:Lyn 4
41	42	43	44	45
500	500	500	500	500
20	20	20	20	20
	126.9	126.9	153.9	81.4
	undigested	apaI	apaI	apaI
	cutsmart	cutsmart	cutsmart	cutsmart
41	42	43	44	45
#DIV/0!	3.94	3.94	3.25	6.14
2	2	2	2	2
1	1	1	1	1
4/5	4/6	4/7	4/8	4/9
#DIV/0!	13.1	13.1	13.8	10.9
41	42	43	44	45
4	4	4	4	4
	bad digest	bad digest	bad digest	bad digest

A/B	A/B4	A4/B	A4/B4	TRE:CD79A	TRE:CD79B	TRE:GmabL	hef1a:Lyn	TRE:GmabH	TRE:Lyn-mkate

tube name	Hyper Ladder 1kb	A/B 1	A/B 1	A/B 2	A/B 3	A/B 4	A/B 5	A4/B 1	A4/B 1	A4/B 2	A4/B 3	A4/B 4	A4/B 5	A/B4 1	A/B4 1	A/B4 2	A/B4 3	A/B4 4	A/B4 5	Hyper Ladder 1kb	Hyper Ladder 1kb	A4/B4 1	A4/B4 1	A4/B4 2	A4/B4 3	A4/B4 4	A4/B4 5	TRE:CD79A	TRE:CD79A	TRE:CD79B	TRE:CD79B	TRE:GmabL	TRE:GmabL	TRE:GmabH 1	TRE:GmabH 1	TRE:GmabH 2	TRE:GmabH 3	TRE Lyn-mKate	TRE Lyn-mKate	Hyper Ladder 1kb	Hyper Ladder 1kb	hef1a:Lyn 2	hef1a:Lyn 2	hef1a:Lyn 3	hef1a:Lyn 4
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45
DNA mass desired (ng)	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500	500
total volume(ul)	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20	20
DNA concentration (ng/ul)		154.9	154.9	441.6	321.3	242.1	332.2	432.6	432.6	537.4	484.5	488.6	478.3	229.5	229.5	153.7	379.2	490.6	270.6			451.2	451.2	88	481.9	136.9	110.7	174.4	174.4	223	223	184	184	131	131	154.1	185.4	277	277			126.9	126.9	153.9	81.4
Enzyme Name		undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI	undigested	NsiI	NsiI	NsiI	NsiI	NsiI			undigested	NcoI	NcoI	NcoI	NcoI	NcoI	undigested	StuI	undigested	BamHI	undigested	BamHI	undigested	StuI	StuI	StuI	undigested	bglI			undigested	apaI	apaI	apaI
Buffer Name		3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1	3.1			3.1	3.1	3.1	3.1	3.1	3.1	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	cutsmart	3.1	3.1			cutsmart	cutsmart	cutsmart	cutsmart
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45
DNA volume	#DIV/0!	3.23	3.23	1.13	1.56	2.07	1.51	1.16	1.16	0.93	1.03	1.02	1.05	2.18	2.18	3.25	1.32	1.02	1.85	#DIV/0!	#DIV/0!	1.11	1.11	5.68	1.04	3.65	4.52	2.87	2.87	2.24	2.24	2.72	2.72	3.82	3.82	3.24	2.70	1.81	1.81	#DIV/0!	#DIV/0!	3.94	3.94	3.25	6.14
buffer volume	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
enzyme volume	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1	1
strip tube #	1/1	1/2	1/3	1/4	1/5	1/6	1/7	1/8	1/9	1/10	1/11	1/12	2/1	2/2	2/3	2/4	2/5	2/6	2/7	2/8	2/9	2/10	2/11	2/12	3/1	3/2	3/3	3/4	3/5	3/6	3/7	3/8	3/9	3/10	3/11	3/12	4/1	4/2	4/3	4/4	4/5	4/6	4/7	4/8	4/9
water volume	#DIV/0!	13.8	13.8	15.9	15.4	14.9	15.5	15.8	15.8	16.1	16.0	16.0	16.0	14.8	14.8	13.7	15.7	16.0	15.2	#DIV/0!	#DIV/0!	15.9	15.9	11.3	16.0	13.3	12.5	14.1	14.1	14.8	14.8	14.3	14.3	13.2	13.2	13.8	14.3	15.2	15.2	#DIV/0!	#DIV/0!	13.1	13.1	13.8	10.9
gel/lane	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45
loading dye	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4	4
results
notes		digested						digested					Ran out of NsiI

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Gels 2014-08-21