BBa_I20260 (promoter+GFP) (J23101-B0032-E0040-B0015) in the pSB3K3 vector - kanamycin resistance
BBa_J23101 (constitutive promoter) + BBa_E0240 (GFP) (B0032-E0040-B0015), must be built in the pSB1C3 backbone - chloramphenicol resistance
BBa_J23115 (constitutive promoter) + BBa_E0240 (GFP) (B0032-E0040-B0015), must be built in the pSB1C3 backbone - chloramphenicol resistance
Use flow cytometry to measure fluorescence output. Must calibrate cytometer with fluorescent beads to get absolute units.
Data must be measured in triplicate (i.e., pick three colonies per plate). Must include mean and standard deviation across samples.