14-Mar-7
LR Reaction:
Label
|
Gene
|
(fmol)
|
Promoter
|
(fmol)
|
Destinatioin
|
(fmol)
|
LR Clonase(uL) |
Total Volume
|
JX 1
|
mKate
|
5
|
Het1a-LacO
|
5
|
1-2
|
10
|
1
|
4
|
JX 2
|
rtTA
|
5
|
Het1a
|
5
|
1-2
|
10
|
1
|
4
|
14-Mar-14
Gibson Reaction Mix
Sample Y166
|
DNA |
I-Sce-1 |
Buffer |
Water |
Total |
Mass (μg) |
1 |
|
|
|
|
Concentration (ng/μL) |
316 |
|
|
|
|
Volume (μL) |
3.16 |
1 |
2 |
13.84 |
20 |
Order |
2 |
4 |
3 |
1 |
|
Created Gibson reactio mix. Reactants entered in volume and order noted in table.
14-Mar-16
Gibson Reaction Mix for All Samples
Mass (μg) |
Enzyme (μL) |
Buffer (μL) |
Total (μL) |
|
1 |
1 |
2 |
20 |
|
|
|
|
|
|
|
Con (ng/μL) |
Vol DNA (μL) |
Vol H2O (μL) |
|
1.1 |
151 |
6.62 |
10.38 |
CMU-Nde1 |
1.2 |
267 |
3.75 |
13.25 |
|
1.3 |
141 |
7.09 |
9.91 |
|
2.1 |
652 |
1.53 |
15.47 |
TRE-t-Sta1 |
2.2 |
263 |
3.80 |
13.20 |
|
2.3 |
212 |
4.72 |
12.28 |
|
4.1 |
149 |
6.71 |
10.29 |
TRE-t-Sta1 |
4.2 |
415 |
2.41 |
14.59 |
|
4.3 |
583 |
1.72 |
15.28 |
|
6.1 |
353 |
2.83 |
14.17 |
TRE-t-Sta1 |
6.2 |
593 |
1.69 |
15.31 |
|
6.3 |
361 |
2.77 |
14.23 |
|
7.1 |
448 |
2.23 |
14.77 |
Het1a-Xhol |
7.2 |
237 |
4.22 |
12.78 |
|
7.3 |
186 |
5.38 |
11.62 |
|
8.1 |
246 |
4.07 |
12.93 |
Het1a-Lac-BamH1 |
8.2 |
182 |
5.49 |
11.51 |
|
8.3 |
871 |
1.15 |
15.85 |
|
9.1 |
410 |
2.44 |
14.56 |
TRE-t-Sta1 |
9.2 |
214 |
4.67 |
12.33 |
|
9.3 |
185 |
5.41 |
11.59 |
|
10.1 |
285 |
3.51 |
13.49 |
CMU-Nde1 |
10.2 |
547 |
1.83 |
15.17 |
|
10.3 |
388 |
2.58 |
14.42 |
|
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Gel Figure for Previous Samples: