• Created by Unknown User (gary_9@mit.edu), last modified by Unknown User (lylaatta@mit.edu) on Jun 13, 2014 10:10

Subgroup Specific:

miRNA: 

  • Contact Jake Beal about construction of miRNA sensors 
  • Look into cell lines 
    • we already have: HEK293, Cho, Hela, ovarian cancer cells, breast cancer cells
    • Ask Samira about what cell lines she is thinking of getting 
  • Get more information about the miRNA 
  • Ensure that the profile is exactly the one you want in preparation for ordering

Treatment:

  • Anaspec contacted about getting a smaller BACE2 assay kit; they said they do not scale down the kit
    • Look into getting kit at a discounted price 
  • Practice LR reaction done in preparation for working with BACE2 
  • Talk to someone about making miRNA for BACE1 inhibition (Samira or Kyle)

Antibody:

  • Gblocks ordered 
  • Ran through experimentation with Brian - experimental procedure "finalized' 
  • More research to be done about family of proteins that appear to be recruited 
  • Look into other sources of Igm so that experimentation can be started earlier 
  • Josh Leonard Northwestern - talk to him about the cell surface receptors he is working on 

Receptor:

  • Put plasmids into parts list

General: 

  • Parts list - look at last year's method of sorting
    • Put plasmids on to that list  

Plasmid Progress: on Parts List by 3pm: 

  • miRNA:
    • meeting with Jeremy about plasmids for high and low sensors 
  • Treatment: 
    • waiting BACE2 + primers 
    • looking into BACE1 plasmids (pENTR and miRNA) 
    • TRE + BACE2 plasmid 
    • BACE2-YFP pENTR (C terminal fusion designed, N terminal fusion should be easy to design)
    • Notes 
      • intronic w/ mKate
      • U6 promoter 
      • U6 teto promoter regulated with tetR 
  • Antibodies: 
    • Gantenerumab (Gmab): we have heavy and light chain gBlocks 
    • CD79A/B: we have pENTR 
    • Reporter circuit: we have from Valia 
    • Lyn: we have pENTR
      • Look into point mutating it for autophosphorylation
      • EYFP fusion
    • Syk: we have pENTR
      • look into point mutating it 
      • fusion with EYFP and TEVp 
    • TEV related plasmids that we have (talk to Nevin): 
      •  Gal4UAS-EYFP
      • Gal4UAS-mKate
      • TRE-TEVp
      • Hef1a-rtTa
      • TRE-Syk-TEVp
      • TRE-EBFP-TEVp
    • G-mab heavy chain - Tcs - Gal4VP16 fusion being designed 
    • CD79A/B - Tcs - Gal4VP16 being designed
  • Receptors:
    • Have: pENTR: LilrB2, PirB, Hef1a
    • Designed: LilrB2/PirB-EBFP

 

 

 

 

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