• Created by Unknown User (aleffell@mit.edu), last modified by Unknown User (teague@mit.edu) on Aug 04, 2014 12:50
DatePrimer NumberDescriptionDesigner(s)Instructor CheckLocation
6/11AL_1BACE2_Primer_FALordered 06/123rd floor
6/11AL_2BACE2_Primer_R

AL

ordered 06/123rd floor
6/11AL_3YFP_Primer_FALordered 06/123rd floor
6/11AL_4YFP_Primer_FALordered 06/123rd floor
6/16JA_1YFP primerFJA

ordered 06/17

 
6/16JA_2YFP primerRJAordered 06/17 
6/16JA_7YFP primerFJAordered 06/17 
6/16JA_8YFP primerRJAordered 06/17 
6/16iGEM 020EYFP(NTerm)_Primer_RALordered 06/17 
6/16iGEM 021EYFP(NTerm)_Primer_FALordered 06/17 
6/16iGEM 022 BACE1(NTerm)_Primer_RALordered 06/17 
6/16iGEM 023BACE1(NTerm)_Primer_FALordered 06/17 
6/17iGEM 011PirB primer reverseSSordered 06/17 
6/17iGEM 010PirB primerSSordered 06/17 
6/17iGEM 009LilrB2 primer reverseSSordered 06/17 
6/17iGEM 008LilrB2 pENTR primerSSordered 06/17 
6/17iGEM 007EBFP2 primer reverseSSordered 06/17 
6/17iGEM 006EBFP2-pirb primerSSordered 06/17 
6/17iGEM 005EBFP2-lilrb2  primerSSordered 06/17 
6/17iGEM 001-2BACE2_Primer_F (updated tm)ALordered 06/18 
6/17iGEM 002-2BACE2_Primer_R (updated tm)ALordered 06/18 
6/17iGEM 003-2YFP_Primer_F (updated tm)ALordered 06/18 
6/17iGEM 004-2YFP_Primer_F (updated tm)ALordered 06/18 
6/18iGEM KRB 001 FTCS-Gal4VP16 forward primerKBordered 06/18 
6/18iGEM KRB 002 RTCS-Gal4VP16 reverse primerKBordered 06/18 
6/18iGEM KRB 003 FCD79A forward primerKBordered 06/18 
6/18iGEM KRB 004 RCD79A reverse primer with stop codonKBordered 06/18 
6/18iGEM KRB 005 RCD79A reverse primer for fusion with TEV cleavage siteKBordered 06/18 
6/18iGEM CR 001 FSyk Sequencing Primer 1CRordered 06/18 
6/18iGEM CR 002 FSyk Sequencing Primer 2CRordered 06/18 
6/18iGEM CR 003 FSyk Sequencing Primer 3CRordered 06/18 
6/18iGEM CR 004 FLyn Sequencing Primer 1CRordered 06/18 
6/18iGEM CR 005 FLyn Sequencing Primer 2CRordered 06/18 
6/18iGEM 014 BACE1 primer forwardfor BACE1-eYFP entry vector (corrected Tm)JAordered 06/17 
6/18iGEM 015 BACE1 primer reversefor BACE1-eYFP entry vector (corrected Tm)JAordered 06/17 
6/18iGEM 016 BACE2 primer forwardfor eYFP-BACE2 entry vector (corrected Tm)JAordered 06/17 
6/18iGEM 017 BACE2 primer reversefor eYFP-BACE2 entry vector (corrected Tm)JAordered 06/17 
6/18iGEM_AS001 CD79B Q1_FCD79B forward primerAS

ordered 06/18

 
6/18iGEM_AS002 CD79B QX_stop_RCD79B reverse primer with stop codonASordered 06/18 
6/18iGEM_AS003 CD79B Qlinker_RCD79B reverse primer with TCS linker overhangASordered 06/18 
6/18iGEM_AS004 Syk Q1_FSyk forward primerASordered 06/18 
6/18iGEM_AS005 Syk QX_stop_RSyk reverse primer with stop codonASordered 06/18 
6/18iGEM2014-EE-003-RSyk F for syk-15-tevpEEordered 06/18 
6/18iGEM2014-EE-004-FSyk F for syk-15-tevpEEordered 06/18 
6/18iGEM2014-EE-005-Ftev R for syk-15-tevpEEordered 06/18 
6/18iGEM2014-EE-006-Rtev R for syk-15-tevpEEordered 06/18 
6/18iGEM KRB 006 FmKate forward primer with linkerKBordered 06/18 
6/18iGEM KRB 007 RmKate reverse primerKBordered 06/18 
6/18iGEM KRB 008 FeYFP forward primer with linkerKBordered 06/18 
6/18iGEM KRB 009 ReYFP reverse primerKBordered 06/18 
6/18iGEM KRB 010 RSyk reverse primer with linkerKBordered 06/18 
6/18iGEM KRB 011 RLyn reverse primer with linkerKBordered 06/18 
6/19iGEM 018 LilrB2 pENTR primer (fixed)Previous primer had Kozak sequence missingSSordered 06/20 
6/19iGEM_AS006 Syk Q1_FSyk forward primerASordered 06/20 
6/19iGEM_AS007 Syk Qlonglink_RSyk reverse primer with 15aa linker overhangASordered 06/20 (modified BT) 
6/19iGEM_AS008 Lyn Q1_FLyn forward primerASordered 06/20 
6/19iGEM_AS009 Lyn Qlonglink_RLyn reverse primer with 15aa linker overhangASordered 06/20 
6/19iGEM_AS010 TEVp Qlonglink_FTEVp forward primer with 15aa linker overhangASordered 06/20 
6/19iGEM_AS011 TEVp QX_RTEVp reverse primerASordered 06/20 
6/22iGEM CR 006 F (Lyn PCR Primer)pDONR223-LynCR

ordered 06/23

 
6/22iGEM CR 007 R (Lyn PCR Primer)pDONR223-LynCRordered 06/23 
6/25iGEM_AS012 CD79B Q1_FCD79B forward primerAS

ordered 06/27

 
6/25iGEM_AS013 CD79B QX_stop_RCD79B reverse primer with stop codonASordered 06/27 
6/25iGEM_AS014 CD79B Qlinker_RCD79B reverse primer with TCS linker overhangASordered 06/27 
6/26iGEM_Q1_mir-144-5p_mir-181c-5p_Q2_F2 input low sensor ultramer for mir-144-5p & mit-18c-5pGB

can't order as a gblock

 
6/27iGEM_SS001PirB 3,466 FSSordered 06/27 
6/27iGEM_SS002PirB 3,920 FSSordered 06/27 
6/27iGEM_SS003PirB 4,404 FSSordered 06/27 
6/27iGEM_SS004PirB 2,909 FSSordered 06/27 
6/27iGEM_SS005LilrB2 2,915 FSSordered 06/27 
6/27iGEM_SS006LilrB2 3,480 FSSordered 06/27 
6/27iGEM Mature 30dsiRNA sequence for 30dGB

ordered 07/03

 
6/27iGEM Mature125bsiRNA sequence for 125bGBordered 07/03 
6/27

iGEM Mature 144

siRNA sequence for 144GBordered 07/03 
6/27iGEM Mature 146asiRNA sequence for 146aGBordered 07/03 
6/27iGEM Mature 181csiRNA sequence for 181cGBordered 07/03 
6/27iGEM Mature let-7fsiRNA sequence for let-7fGBordered 07/03 
7/1SeqPrimer_pENTR_eYFP_BACE1 - 3,017 Fsequencing primer for pENTR_eYFP_BACE1ALordered 07/01 
7/1SeqPrimer_pENTR_eYFP_BACE1 - 4,140 Fsequencing primer for pENTR_eYFP_BACE1ALordered 07/01 
7/1SeqPrimer_pENTR_eYFP_BACE1 - 4,701 Fsequencing primer for pENTR_eYFP_BACE1ALordered 07/01 
7/1SeqPrimer_pENTR_eYFP_BACE1_- 3,615 Fsequencing primer for pENTR_eYFP_BACE1ALordered 07/01 
7/1

iGEM CR 008 F

Gmab H Sequencing Primer 1 of 3

CR

ordered 07/01

 
7/1iGEM CR 009 FGmab H Sequencing Primer 2 of 3CRordered 07/01 
7/1iGEM CR 010 FGmab H Sequencing Primer 3 of 3CRordered 07/01 
7/1iGEM CR 011 FLyn Sequencing Primer 1 of 3CRordered 07/01 
7/1iGEM CR 012 FLyn Sequencing Primer 2 of 3CRordered 07/01 
7/1iGEM CR 013 FLyn Sequencing Primer 3 of 3CRordered 07/01 
7/1iGEM CR 014 FmKate Fusion Primer 1 of 1CRordered 07/01 
7/1iGEM CR 015 F TEV Fusion Sequencing Primer 1 of 1CRordered 07/01 
7/1iGEM CR 016FeYFP Fusion Sequencing Primer 1 of 1CRordered 07/01 
7/1

iGEM CR 017 F

TEV Fusion Primer 1CRordered 07/01 
7/3iGEM 001v2BACE2 N-term FALordered 07/03 
7/3iGEM 002v2BACE2 N-term RALordered 07/03 
7/3iGEM 003v2YFP C-term2 FALordered 07/03 
7/3iGEM 004V2YFP C-term2 RALordered 07/03 
7/3iGEM 012v2YFP C-term1 FALordered 07/03 
7/3iGEM 013v2YFP C-term1 RALordered 07/03 
7/3iGEM 014v2BACE1 N-term FALordered 07/03 
7/3iGEM 015v2BACE1 N-term RALordered 07/03 
7/3iGEM 016v2BACE2 C-term FALordered 07/03 
7/3iGEM 017v2BACE2 C-term RALordered 07/03 
7/3iGEM 018v2YFP N-term2 FALordered 07/03 
7/3iGEM 019v2YFP N-term2 RALordered 07/03 
7/3Lyla PirB pENTR sequencing primer 1PirB pENTR sequencing primerLAdon't need 07/08 
7/3Lyla PirB pENTR sequencing primer 2PirB pENTR sequencing primerLAdon't need 07/08 
7/3Lyla PirB pENTR sequencing primer 3PirB pENTR sequencing primerLAdon't need 07/08 
7/3Lyla PirB pENTR sequencing primer 4PirB pENTR sequencing primerLAdon't need 07/08 
7/3Lyla LilrB2 pENTR sequencing primer 1LilrB2 pENTR sequencing primerLAdon't need 07/08 
7/3Lyla LilrB2 pENTR sequencing primer 2LilrB2 pENTR sequencing primerLAdon't need 07/08 
7/3Lyla LilrB2 pENTR sequencing primer 3LilrB2 pENTR sequencing primerLAdon't need 07/08 
7/3

iGEM AL 005

 

AL

ordered 07/03 
7/3iGEM AL 006 ALordered 07/03 
7/3iGEM AL 007 ALordered 07/03 
7/3iGEM AL 008 ALordered 07/03 
7/7iGEM AL1FW_m01ALordered 07/07 
7/7iGEM AL2RV_m01ALordered 07/07 
7/7iGEM AL3FW_m02ALordered 07/07 
7/7iGEM AL4RV_m02 ALordered 07/07 
7/7iGEM AL5FW_m03ALordered 07/07 
7/7iGEM AL6RV_m03 ALordered 07/07 
7/7iGEM AL7FW_m04 ALordered 07/07 
7/7iGEM AL8RV_m04 >60bpALordered 07/07 
7/7iGEM AL9FW_m05ALordered 07/07 
7/7iGEM AL10RV_m05 ALordered 07/07 
7/7iGEM AL11FW_m06ALordered 07/07 
7/7iGEM AL12RV_m06 ALordered 07/07 
7/7iGEM AL13FW_m07ALordered 07/07 
7/7iGEM AL14RV_m07 ALordered 07/07 
7/7iGEM AL15FW_m08ALordered 07/07 
7/7iGEM AL16RV_m08 ALordered 07/07 
7/7iGEM AL17FW_m09ALordered 07/07 
7/7iGEM AL18RV_m09 ALordered 07/07 
7/7iGEM AL19FW_m10ALordered 07/07 
7/7iGEM AL20RV_m10 ALordered 07/07 
7/8iGEM AL010BACE2 Sequencing Primer 1ALdon't need 07/08 
7/8iGEM AL011BACE2 Sequencing Primer 2ALordered 07/08 
7/8iGEM AL012BACE2 Sequencing Primer 3ALdon't need 07/08 
7/8iGEM AL013BACE1 Sequencing Primer 1ALdon't need 07/08 
7/8iGEM AL014BACE1 Sequencing Primer 2ALordered 07/08 
7/8iGEM AL015BACE1 Sequencing Primer 3ALdon't need 07/08 
7/9iGEM_SS007 Cofilin FEYFP_SGLinker_CofilinSSdon't need 7/10 
7/9iGEM_SS008 Cofilin REYFP_SGLinker_CofilinSSdon't need 7/10 
7/9iGEM_SS009 EYFP_Cofilin FEYFP_SGLinker_CofilinSSdon't need 7/10 
7/9iGEM_SS010 EYFP_Cofilin REYFP_SGLinker_CofilinSSdon't need 7/10 
7/10iGEM Q1_mir-144-5p_Q3 Flow sensor ultramersGBordered 07/10 
7/10iGEM Q3_mir-181c-5p_Q2 Flow sensor ultramersGBordered 07/10 
7/10iGEM2014-EE-008point mutation 1EElet's hold off 07/10 
7/10 iGEM2014-EE-009 point mutation 2 a1145aEElet's hold off 07/10 
7/10 iGEM2014-EE-010 point mutation 3 a1481a_EElet's hold off 07/10 
7/10 iGEM2014-EE-011 point mutation 4 a1145a_antisenseEElet's hold off 07/10 
7/10iGEM_SS011Cofilin F (C) YFP fusionSSordered 07/10 
7/10iGEM_SS012Cofilin R (C) YFP fusionSSordered 07/10 
7/10iGEM_SS013LilrB2 F (N) YFP fusionSSordered 07/10 
7/10iGEM_SS014LilrB2 R (N) YFP fusionSSordered 07/10 
7/10iGEM_SS015PirB F (N) YFP fusionSSordered 07/10 
7/10iGEM_SS016PirB R (N) YFP fusionSSordered 07/10 
7/11GEM_miR-low_SequencingSequencing primer for miR-low sensor regionJXordered 07/11 
7/11iGEM KRB 012 RPrimer for amplifying 1st half of Lyn (for use with gBlock)KRBordered 07/12 
7/11iGEM KRB 013 FTEVp (for use with Lyn-fusion gBlock)KRBordered 07/12 
7/11iGEM KRB 014 FmKate (for use with Lyn-fusion gBlock)KRBordered 07/12 
7/11iGEM KRB 015 FLyn (AarI)KRBordered 07/12 
7/11iGEM KRB 016 RLyn+stop (AarI)KRBordered 07/12 
7/11iGEM KRB 017 F8aa_linker-mKate (AarI)KRBordered 07/12 
7/11iGEM KRB 018 RmKate (AarI)KRBordered 07/12 
7/11iGEM KRB 019 RLyn-8aa_linker (AarI)KRBordered 07/12 
7/11iGEM KRB 020 F15aa_linker-TEVp (AarI)KRBordered 07/12 
7/11iGEM KRB 021 RTEVp (AarI)KRBordered 07/12 
7/11iGEM KRB 022 RLyn-15aa_linker (AarI)KRBordered 07/12 
7/11iGEM KRB 023 FSyk (AarI)KRBwait 
7/11iGEM KRB 024 RSyk-8aa_linker (AarI)KRBwait 
7/11iGEM KRB 025 F8aa_linker-eYFP (AarI)KRBwait 
7/11iGEM KRB 026 ReYFP (AarI)KRBwait 
7/14iGEM_SS017 LilrB2 R N TCS G4 7.14 SSordered 07/14 
714iGEM_SS018 PirB R N TCS G4 7.14 SSordered 07/14 
7/16iGEM AL21F SP B1YAL  
7/16iGEM AL22R SP B12YAL  
7/16iGEM AL23F SP B2YAL  
7/16iGEM AL24R SP YB1AL  
7/16iGEM AL25F SP YB12AL  
7/16iGEM AL26R SP YB2AL  
7/24iGEM2014EE010Rattaches first part of ggsggsgg to CD79BEE

not ordered

 
7/24iGEM2014EE011Fattaches second half og ggsggsgg to start of ebfpEEnot ordered 
7/24iGEM2014EE012Rend of eBFP and QxEEnot orderer 
7/24iGEM2014EE013Rattaches first part of ggsggsgg to cd79bEEnot ordered 
7/27iGEM Mature 30d sensesiRNA sequence for 30d

GB

ordered 
7/27iGEM Mature125b sensesiRNA sequence for 125bGBordered 
7/27

iGEM Mature 144 sense

siRNA sequence for 144GBordered 
7/27iGEM Mature 146a sensesiRNA sequence for 146aGBordered 
7/27iGEM Mature 181c sensesiRNA sequence for 181cGBordered 
7/27iGEM Mature let-7f sensesiRNA sequence for let-7fGBordered 
8/02iGEM_SS_802 YFP C FYFP forward primer without gnarly hairpins (smile)SSordered BT 08/04 

 

 

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