Derived from the Abcam website: http://www.abcam.com/amyloid-beta-peptide-1-42-human-ab120301.html

  1. In a fume hood, resuspend the polypeptide at a concentration of 1 mg/ml in neat HFIP (1,1,1,3,3,3-hexafluoro-2-propanol).
  2. Incubate at room temperature for 1 hour, vortexing briefly every 10 minutes at a moderate speed.\
  3. Sonicate in a sonicating water bath on high for 10 minutes.
  4. In a fume hood, place the HFIP/peptide solution under a gentle stream of dry nitrogen gas.
  5. Resuspend in ????? neat DMSO.  Incubate at room temperature for 12 minutes.
  6. Aliquot ????? volumes into individual 2 ml microcentrifuge tubes.  Store at -80 degC.

Oligomerization

  1. Retrieve a single aliquot from the -80.  Resuspend in ????? ul PBS for a final concentration of ????. 
  2. Incubate 2 hours at room temperature to allow for peptide aggregation.
  • No labels