THINGS TO DO

Task	Person	Date
Syk-TEVp cleavage with serial dilutions Transfect Add dox Cytometry	CR
BCR membrane localization with serial dilution	CR
Cofilin Western Blot Run gel and trasnfer Antibody incubations and imaging	LA	8/26 8/27
PirB abeta binding live microscopy Stain and prepare for microscopy (5pm)	LA	8/27
LilrB2 localization Seed Transfect Reseed Stain	LA	9/4 9/5 9/6 9/7
LilrB2 abeta binding microscopy Seed Transfect Reseed Stain	LA	9/4 9/5 9/6 9/7
PirB abeta binding microscopy Seed Transfect Reseed Stain	LA	9/4 9/5 9/6 9/7
TEVp-Cofilin cleavage Transfect Prepare for cytometry	LA
L7ae dilution series	GB
BACE1 and BACE2 localization	JA
BACE1 and BACE2 assay	JA
Multi Input miRNA Sensors	GB

Experiment to be Analyzed	Person
miRNA L7Ae Sensors (w w/out siRNA)	JX, GB
multi input sensors	GB 9.19.14

Task	Person	Date
eBFP streak plate from 2013 stock pick colonies midiprep restriction verify		9/7 9/8 9/9 9/10
Lyn-TEVp
BACE1 YFP fusion LR Kill and transform Pick colonies Miniprep Verify pEXPR Midiprep	JA
BACE2 YFP fusion LR Kill and transform Pick colonies Miniprep Verify pEXPR Midiprep	JA
BCR Gibson	KRB
LilrB2-YFP Golden gate Transform Pick colonies Miniprep Run gel Send for sequencing (if gel appears right) LR Kill and transform Pick colonies Miniprep Run gel Innoculate midi (if gel appears right) Midiprep	SS	8/26 8/27 8/28 8/29 8/29 8/29 8/29 8/30 8/31 9/1 9/1 9/1 9/2
LilrB2-TCS-Gal4VP16 Golden gate Transform Pick colonies Miniprep Run gel Send for sequencing (if gel appears right) LR Kill and transform Pick colonies Miniprep Run gel Innoculate midi (if gel appears right) Midiprep	SS	8/26 8/27 8/28 8/29 8/29 8/29 8/29 8/30 8/31 9/1 9/1 9/1 9/2

Task	Person	Date
External Wiki	Tsar: ? JX (willing to help)
Survey analysis	AG
Novartis person (what do we want from her?)

WEEKLY TISSUE CULTURE DUTIES
M	T	W	R	F	Sa	Su
Gary	Kathryn	Lyla	James	Alexa	Christian	Christian

Things to do