Results
| Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| tube | Hyper Ladder 1kb | GG DONR | pENTRSyk-eYFP 1 | pENTRSyk-eYFP 1 | pENTRSyk-eYFP 2 | pENTRSyk-eYFP 3 | pENTRSyk-eYFP 4 | pENTRSyk-eYFP 5 | pENTR Lyn TEV 1 | pENTR Lyn TEV 1 | pENTR Lyn TEV 2 | pENTR Lyn TEV 3 | pENTR Lyn TEV 4 | pENTR Lyn TEV 5 | GG DONR | Hyper Ladder 1kb |
| enzyme | NsiI | undigested control | NsiI | NsiI | NsiI | NsiI | NsiI | undigested control | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | ||
| Result(P/F) | ||||||||||||||||
| Notes | may not be loaded, there were a lot of bubbles. | scratch in gel on right side |
| Lane | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 |
| Tube | Hyper Ladder 1kb | GG DONR | pENTR Lyn-mKate 1 | pENTR Lyn-mKate 1 | pENTR Lyn-mKate 2 | pENTR Lyn-mKate 3 | pENTR Lyn-mKate 4 | pENTR Lyn-mKate 5 | pENTR Lyn-stop 1 | pENTR Lyn-stop 1 | pENTR Lyn-stop 2 | pENTR Lyn-stop 3 | pENTR Lyn-stop 4 | pENTR Lyn-stop 5 | Hyper Ladder 1kb |
| Enzyme | PvuII | undigested control | PvuII | PvuII | PvuII | PvuII | PvuII | undigested control | PvuII | PvuII | PvuII | PvuII | PvuII | ||
| Result(P/F) |
Old gel on the right for comparison
Virtual gels from Geneious
Fail state (ggDONR) | ||||
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