Results
| Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| tube | Hyper Ladder | GG DONR | pENTRSyk-eYFP 1 | pENTRSyk-eYFP 2 | pENTRSyk-eYFP 3 | pENTRSyk-eYFP 4 | pENTR Syk tev 1 | pENTR Syk tev 2 | pENTR Syk tev 3 | pENTR Syk tev 4 | Syk+stop 1 | Syk+stop 2 | Syk+stop 3 | Syk+stop 4 | Syk+stop 5 | pENTR Lyn TEV 1 | pENTR Lyn TEV 2 | pENTR Lyn TEV 3 | pENTR Lyn TEV 4 | Hyper Ladder |
| enzyme | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | NsiI | PvuII | PvuII | PvuII | PvuII | ||
| Result(P/F) | F | F | F | F | P | P | P | F | F | P | F | F | F | F | F | F | F | |||
| Notes | looks like Q to Q ligation | ggDONR | ggDONR | ggDONR | ggDONR |
| Lane | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
| Tube | Hyper Ladder | GG DONR | pENTR Lyn-mKate 1 | pENTR Lyn-mKate 2 | pENTR Lyn-mKate 3 | pENTR Lyn-mKate 4 | pENTR Lyn-stop 1 | pENTR Lyn-stop 2 | pENTR Lyn-stop 3 | pENTR Lyn-stop 4 | CD79A-tcs-gal4 1 | CD79A-tcs-gal4 2 | CD79A-tcs-gal4 3 | CD79A-tcs-gal4 4 | CD79A-tcs-gal4 5 | CD79A-stop 1 | CD79A-stop 2 | CD79A-stop 3 | CD79A-stop 4 | CD79A-stop 5 |
| Enzyme | PvuI | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | PvuII | |
| Result(P/F) | F | F | F | F | F | F | F | F | P | P | F | F | P | F | F | F | P | F |
Virtual gels from Geneious
