- Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation
- Five amino acids produced limited hydrogen but abundant SMPs in dark-fermentation.
- C conversion efficiencies of alanine and serine were 85.3–94.1% in dark-fermentation.
- Dark-fermentation effluent of alanine gave a high H2 yield in photo-fermentation.
- HVCE of alanine to hydrogen by combined dark- and photo-fermentation was 25.1%.
- HPB (hydrogen producing bacteria): Clostridium butyricum
- Dump pure amino acids and HPB into a dark reactor (35 C)
- Separate residual solutions of dark fermentation through centrifuging
- PSB (photosynthetic bacteria): Rhodopseudomonas palustris
- Dilute treated supernatant and add to autoclaved enrichment medium with PSB and put into photo-fermentation reactor (30 C)
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