Culture Collection_Personal

Please simply list-up the microbial strains you have.

EXCEL work sheet can be copied & Pasted.

Filippo

Species	Strain
E.coli (background K12) --> BL1	HCB33 (TSS496): E. coli wild type HCB1584: E. coli D(tar) HCB268: E. coli tsr::Tn5-1a TSS945: E. coli D(tsr) UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer) UU2162: D(tar tap) D(tsr) D(trg) D(aer) TSS584: HCB33 / pVS129 (ecfp on pTrc99A) TSS585: HCB33 / pVS132 (eyfp on pTrc99A) TSS586: HCB33 / mCherry on pTrc99A
E. coli (background DH5a) --> BL1	TSS379 TSS342 TSS270 TSS712 TSS711 TSS943 TSS694 HCB1666
Salmonella (background LT2) --> BL2 (attenuated)	LT2 (TSS495): Salmonella LT2 wild type LT2 (TSS497): Salmonella LT2wild type pSLT cured TSS511: Salmonella LT2 D(cheV) TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33 TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt) TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc) LT2 RecA: strain with the recA gene inactivated. kanamycin resistance is inserted in the mutated site. LT2 recAo: strain with the SOS box mutated. Chloramphenicol resistance is inserted in the mutated site. LT2 LexA(Ind-): strain with the lexA gene mutated. Chloramphenicol resistance is inserted in the mutated site. LT2 CheY: strain with the cheY gene inactivated. Chloramphenicol resistance is inserted in the mutated site. LT2 CheB: strain with the cheB gene inactivated. Chloramphenicol resistance is inserted in the mutated site. TSS587: TSS497 / pVS129 (ecfp on pTrc99A) TSS588: TSS497 / pVS132 (eyfp on pTrc99A) TSS589: TSS497 / mCherry on pTrc99A
Salmonella (background Salmonella 14028) --> BL2	MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip
Escherichia coli (WT)	RP437 (from Linda Turner, Berg's Lab)

Melissa

Name	Strain	Media to use	Location
Vibrio coralliilyticus	BAA-450 (directly from ATCC; used for ISME J 2014 paper)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	P1 GFP (from Joe Pollock/David Bourne)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB1 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB2 wild type (from Eugene Rosenberg by way of Orr Shapiro/Assaf Vardi-- in principle should be same as ATTC type strain)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Stocker 01
Vibrio coralliilyticus	YB1 dsRed (from Orr Shapiro/Assaf Vardi)	2216 + 50ug/ml Kan	Stocker 01
Vibrio coralliilyticus	YB2 dsRed (from Orr Shapiro/Assaf Vardi)	2216 + 50ug/ml Kan	Stocker 01
Vibrio coralliilyticus	Non-motile (fla knockout) YB2 (from Dalit Meron-- sent to Rory while in Stocker lab)	2216 + 100ug/ml Kan (though 50ug should be OK too-- they were originally isolated on 200ug/ml selective medium)	Rory&Mel's box
Vibrio fortis	isolated from healthy coral (non-pathogenic; from Rory Welsh/Becky Vega-Thurber)	2216 (or TCBS plates to select for Vibrios if contamination suspected)	Rory&Mel's box
Serratia marcescens	coral pathogen from Katie Patternson's lab via Rory/Becky	2216	Rory&Mel's box
Bacteriovorax	isolated from healthy coral (preys on V.cor and V.fortis; from Rory Welsh/Becky Vega-Thurber)	grows on lawn or culture of vibrio (coralliilyticus or fortis)	Rory&Mel's box
Pseudovibrio sp.	isolated from healthy coral by JB Raina. Produces the antimicrobial compound TDA	2216	Stocker 01

Cherry

Ruegeria pomeroyi DSS-3 strain, in 2216 media, from Mary Ann Moran's lab (March 2014)

Yutaka

		BOX2
A	1	Vibrio cycrotrophicus	1F111	hsp60 confirmed
A	2	Vibrio cycrotrophicus	1F175
A	3	Vibrio cycrotrophicus	1F273	hsp60 confirmed
A	4	Vibrio cycrotrophicus	1F289	hsp60 confirmed
A	5	Vibrio cycrotrophicus	1F53	hsp60 confirmed
A	6	Vibrio cycrotrophicus	1F97	hsp60 confirmed
A	7	Vibrio cycrotrophicus	FF160
A	8	Vibrio cycrotrophicus	FF274
A	9	Vibrio cycrotrophicus	FFA
B	1
B	2	Vibrio cycrotrophicus	ZF14	hsp60 confirmed
B	3	Vibrio cycrotrophicus	ZF205
B	4	Vibrio cycrotrophicus	ZF270	hsp60 confirmed
B	5	Vibrio cycrotrophicus	ZF28
B	6	Vibrio cycrotrophicus	ZF30	hsp60 confirmed
B	7	Vibrio cycrotrophicus	ZF65	hsp60 confirmed
B	8	Vibrio cycrotrophicus	ZF99
B	9

		Box 01	Strain
B	1	Vibrio sp.	12B9 GFP
B	2	Vibrio sp.	12B9RFP
B	3	Vibrio cycrotrophicus	1F53 GFP
B	4	Vibrio cycrotrophicus	1F53 RFP
B	5	Vibrio cycrotrophicus	1F111 GFP
B	6	Vibrio cycrotrophicus	1F111 RFP
B	7	Vibrio cycrotrophicus	ZF270 GFP
B	8	Vibrio cycrotrophicus	ZF270 RFP
B	9
C	1	Vibrio crassostreae	13D11 GFP
C	2	Vibrio crassostreae	13D11 RFP
C	3	Vibrio sp.	ZF223 GFP
C	4	Vibrio sp.	ZF223 RFP
C	5	Vibrio cycrotrophicus	FF274 RFP
C	6
C	7
C	8
C	9
D	1	Vibrio cycrotrophicus	ZF65
D	2	Vibrio cycrotrophicus	ZF270
D	3	Vibrio ordalii	FS144
D	4	Vibrio ordalii	FF93
D	5	Vibrio ordalii	1S257
D	6	Vibrio ordalii	12B9
D	7	(Vibrio cycrotrophicus)	(1F273)
D	8	(Vibrio cycrotrophicus)	(1F175)
D	9	Vibrio cycrotrophicus	1F111
E	1	Vibrio cycrotrophicus	ZF14
E	2	(Vibrio cycrotrophicus)	(1F289)
E	3	(Vibrio cycrotrophicus)	(1F289)
E	4	V. alginolyticus	YM4
E	5	Vibrio cycrotrophicus	ZF30
E	6	Vibrio cycrotrophicus	ZF99
E	7	Vibrio cycrotrophicus	ZF205
E	8	Vibrio cycrotrophicus	ZF28
E	9	Vibrio cycrotrophicus	FF160
F	1	Vibrio cycrotrophicus	1F53
F	2	Vibrio cycrotrophicus	1F97
F	3	Vibrio cycrotrophicus	FF160
F	4
F	5
F	6
F	7
F	8
F	9
F

E.coli (background K12) --> BL1

HCB33 (TSS496): E. coli wild type

HCB1584: E. coli D(tar)

HCB268: E. coli tsr::Tn5-1a

TSS945: E. coli D(tsr)

UU1250: D(tar tap) (tsr::Kan) D(trg) D(aer)

UU2162: D(tar tap) D(tsr) D(trg) D(aer)

TSS584: HCB33 / pVS129 (ecfp on pTrc99A)

TSS585: HCB33 / pVS132 (eyfp on pTrc99A)

TSS586: HCB33 / mCherry on pTrc99A

E. coli (background DH5a) --> BL1

TSS379

TSS342

TSS270

TSS712

TSS711

TSS943

TSS694

HCB1666

E. coli (natural isolates) --> BL2

ECOR2

ECOR12

ECOR13

ECOR18

ECOR19

ECOR21

ECOR27

ECOR29

ECOR31

ECOR32

ECOR33

ECOR37

ECOR45

ECOR48

ECOR50

ECOR51

ECOR62

ECOR68

Salmonella (background LT2) --> BL2 (attenuated)

LT2 (TSS495): Salmonella LT2 wild type

LT2 (TSS497): Salmonella LT2wild type pSLT cured

TSS511: Salmonella LT2 D(cheV)

TSS642 (TH9972): Salmonella LT2 aer-6508::MudJ

TSS505: Salmonella LT2 D(cheY cheZ) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS516: Salmonella LT2 D(cheY cheZ) D(cheV) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS869 Salmonella LT2 D(cheY cheZ) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS879 Salmonella LT2 D(cheY cheZ) D(tar) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS891: Salmonella LT2 D(cheY cheZ) D(tar) D(tsr) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS953: Salmonella LT2 D(cheY cheZ) D(mcpB) D(mcpC) / pVS88 (cheY-yfp cheZ-cfp) / pBAD33

TSS954: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pML18 (tarSt)

TSS880: Salmonella LT2 D(cheY cheZ) D(tar) /pVS88 (cheY-yfp cheZ-cfp) / pVS123 (tarEc)

TSS587: TSS497 / pVS129 (ecfp on pTrc99A)

TSS588: TSS497 / pVS132 (eyfp on pTrc99A)

TSS589: TSS497 / mCherry on pTrc99A

Salmonella (background Salmonella 14028) --> BL2

MB211 (TSS871): Salmonella 14028 Δtsr Δtar Δtrg Δaer ΔmcpB ΔmcpC Δtcp ΔmcpA Δtip

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