Group A: Membrane Localization
Group B: Receptor Affinity for Beta-amyloid
Group C:
...
Levels of Endogenous Cofilin
Group D: Cofilin Recruitment
- Find data on receptor-cofilin dynamics (esp. how long cofilin stays recruited at the membrane)
- pEXPR hEF1a:cofilin-eYFP
- look at structure of cofilin to determine which (N or C) terminus to put the fluorescent protein
- order cofilin gBlock
- look into different types of cofilin
- look for higher intensity at around membrane
- pEXPR hef1a:eBFP
...
Group D: Cofilin + TEV Protease Recruitment?
...
Group E: (Whole System) Cofilin + TEV Protease Recruitment to Receptor + Cleavage Site + Transcription Factor
...