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Pro Tips

  1. Run at the larger scale
  2. ALWAYS kill it with PRoteinase K
  3. Transform 4 uL of the reaction
  4. Use ALL the transformation tricks
  5. Plate ALL of it
  6. ALWAYS run a PUC19 control for transformations

Instructions for newbies:

1.5 uL of 10 fM Dest

1.5 uL of 5 fM promoter

...