TISSUE CULTURE:
- Best Practices
- Preparing media
- Splitting cells & seeding plates
- Transfection (Lipofectamine LTX)
- Transfection (Lipofectamine 2000)
- Transfection (Lipofectamine 3000)
- FACS Cell Prep
- siRNA Transfection.
- Flow Cytometry with primary and secondary antibodies for membrane staining
- Flow Cytometry recommended controls (from abcam)
- Immunostaining for Fluorescent Microscopy
- Immunostaining for Flow Cytometry
- Gelatin Treatment for Glass Plates
- Beta Amyloid Streptavidin Staining Protocol for Cytometry
- Beta Amyloid Streptavidin Staining Protocol for Live Microscopy
- Attaching B-Cells using Poly-L-Lysine
- Freezing and Thawing HEK293s
MISC. LABORATORY PROCEDURES:
- Making a Glycerol Stock
- Making SOC
- Preparing GGDonr plates
- Preparing LB Agar plates
- Western Blot
- Oligomerizing Beta Amyloid
- Autoclave