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Use anti IgM with a florescent fused secondary antibody to test for membrane localization of the BCR complex. |
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This will test whether or not the BCR complex will reach the cell membrane in HEK293 cells. |
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pEXPR hEF1a: Gmab Light pEXPR hEF1a: Gmab Heavy pEXPR hEF1a: CD79A pEXPR hEF1a: CD79B ALSO NEED: Anti IgM primary antibody Florescently tagged yellow secondary antibody B cells from Velia |
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We expect to see clear yellow fluorescence around the blue stained nuclei indicating BCR localization to the HEK cell membrane. This will be corroborated by similar results with B cells. |
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