Use anti IgM with a florescent fused secondary antibody to test for membrane localization of the BCR complex.
Purpose
This will test whether or not the BCR complex will reach the cell membrane in HEK293 cells.
Parts Needed
pEXPR hEF1a: Gmab Light
pEXPR hEF1a: Gmab Heavy
pEXPR hEF1a: CD79A
pEXPR hEF1a: CD79B
ALSO NEED:
Anti IgM primary antibody
Florescently tagged yellow secondary antibody
B cells from Velia
Setup
Well 1
B-Cells
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Expected Results
We expect to see clear yellow fluorescence around the blue stained nuclei indicating BCR localization to the HEK cell membrane. This will be corroborated by similar results with B cells.