Versions Compared

Old Version 4

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

compared with

New Version 5

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

  •  Transform all LRs (hEF1a:PT, hEF1a:LT, hEF1a:TC, TREt:TC)
  •  Pick hEF1a:YC colonies
  •  Miniprep TREt:YC if it worked. Otherwise, wait for Brian. Worked.
  •  Test LilrB2TCSG4 digest in random places. Is the main LilrB2 stock missing a chunk? Preposterous.
  •  PCR C term YFP?

Digest LilrB2TCSG4 with NcoI and PstI seperately.
Digest TREt:YC with ApaI