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- Transform all LRs (hEF1a:PT, hEF1a:LT, hEF1a:TC, TREt:TC)
- Pick hEF1a:YC colonies
- Miniprep TREt:YC if it worked. Otherwise, wait for Brian. Worked.
- Test LilrB2TCSG4 digest in random places. Is the main LilrB2 stock missing a chunk? Preposterous.
- PCR C term YFP? Nope, brian's looking into it.
- Midiprep inoculate TREt:YC
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Digest LilrB2TCSG4 with NcoI and PstI seperately.
Digest TREt:YC with ApaI
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