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PirB: 

5Gal4VP16 mKate 7.8125 nM abeta 6Gal4VP16 mKate 15.625 nM abeta 7Gal4VP16 mKate 15.625 nM abeta 8Gal4VP16 mKate 31.25 nM abeta
Well 1

HEK293

Well 2

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin200ng

Dummy 200ng

Well 3

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin200ng

Gal4UAS:mKate200ng

Well 4

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 Gal4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate200ng

Dummy 200ng

Well 4

HEK293

hEF1a:eBFP 200ng

TREhEF1a:TEVp-Cofilin PirB-TCS-Gal4VP16 200ng

hEF1a:rtTA 200ng

Gal4UAS:mKate200ng

Dummy 200ng0 nM abeta

500 nM a beta

 
Well
7

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-

Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:

mKate 200ng

0nM DOX

Well
8

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-

Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:

mKate 200ng

1nM DOX

Well
9

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-

Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:

mKate 200ng

10nM DOX

Well
10

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-

Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:

mKate 200ng

1000nM DOX

Well 11
Well 12
Well
9
13

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate mKate 200ng

62.5 nM abeta

0nM DOX

500 nM aBeta


Well
10
14

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate mKate 200ng

1nM DOX

125

500 nM

abeta

Well
11
15

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate mKate 200ng

10nM DOX

500 250 nM abeta


Well
12
16

HEK293

hEF1a:eBFP 200ng

hEF1a:PirB-TCS-Gal4VP16 Gal4VP16 200ng

hEF1a:rtTA 200ng

TRE:TEVp-Cofilin 200ng

Gal4UAS:mKate mKate 200ng

10000nM DOX

500 nM

abeta
Well 13
Well 14
Well 15
Well 16


Well 17
Well 18
Well 19
Well 20
Well 21
Well 22
Well 23
Well 24