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| Antigen | MAb name | Peptide available? | gBlocks ready? |
|---|---|---|---|
| Hen egg lysozyme | HyHEL-10 | YesYes | In progress |
| HIV gp120 | 0.5beta | Kind ofYes | Didn't optimize with IDT for gBlocks |
| HIV gp120 | VRC01 | 99.9% sure | In progress |
...
I still have 0.5beta on this page as a backup antibody for gp120. VRC01 is better though because it can recognize gp120 from many different strains. Also, 0.5beta has a recognition sequence that isn't in exactly in commercially-available peptides. There's a one amino acid substitution which hasn't been studied in the literature.
CLONING STRATEGY:
Cloning steps for each heavy and light chain are in Geneious. Amino acid sequences were back-translated using IDT and BsaI cut sites were manually eliminated. IgM heavy chain
BUDGET:
| Item | Cost |
|---|---|
| HyHEL-10 kappa chain gBlock (784 nt) | $149 |
| HyHEL-10 VH gBlock (472 nt) | $89 |
| Hen egg lysozyme (1g) - Merck-Millipore | $39 |
| VRC01 kappa chain gBlock (772 nt) | $149 |
| VRC01 VH gBlock (496 nt) | $89 |
| HIV1 gp120 JRCSF (50ug) - Eenzyme | $259 |
| TOTAL | $774 |
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