Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

STATUS TABLE

 To do's:

• Midiprep (from freezer)
  • S9TEV
  • B15T15
• Golden Gate
  • B9T9
  • B15T9
• LR
  • hEF1a-CuO:Gmab H
  • hEF1a-CuO:Gmab L
  • hEF1a-CuO:CD79A
  • hEF1a-CuO:CD79B
  • hEF1a-CuO:Lyn
• Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)

Non-Lab To Do's 

• Bug Brian about ordering antibody testing supplies

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?