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Cloning steps for each heavy and light chain are in Geneious. Amino acid sequences were back-translated using IDT and BsaI cut sites were manually eliminated. IgM heavy chain Since we had issues with mutations when we ordered the gantenerumab heavy chain as one gBlock and because ordering the entire heavy chain costs ~$
BUDGET:
| Item | Cost |
|---|---|
| HyHEL-10 kappa chain gBlock (784 nt) | $149 |
| HyHEL-10 VH gBlock (472 nt) | $89 |
| Hen egg lysozyme (1g) - Merck-Millipore | $39 |
| VRC01 kappa chain gBlock (772 nt) | $149 |
| VRC01 VH gBlock (496 nt) | $89 |
| HIV1 gp120 JRCSF (50ug) - Eenzyme | $259 |
| TOTAL | $774 |
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