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Primers for the mIgM PCR are in the "IgM heavy chain primers" folder. The primers don't look like they should cause us too much trouble (some homo/heterodimerization issues but it should be manageable)...
BUDGET:
| Item | Cost |
|---|---|
| HyHEL-10 kappa chain gBlock (784 nt) | $149 |
| HyHEL-10 VH gBlock (472 nt) | $89 |
| Hen egg lysozyme (1g) - Merck-Millipore | $39 |
| VRC01 kappa chain gBlock (772 nt) | $149 |
| VRC01 VH gBlock (496 nt) | $89 |
| HIV1 gp120 JRCSF (50ug) - Eenzyme | $259 |
| TOTAL | $774 |
...