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Adherent Transfection

 HEK293

hEF1a:rtTA 2ng

TRE:Gal4VP16 2ng

UAS:eYFP 2ng

hEF1a:mKate 2ng

hEF1a:eBFP 2ng

 

1000 nm Dox

hEF1a:rtTA 5ng

TRE:Gal4VP16 5ng

UAS:eYFP 5ng

hEF1a:mKate 5ng

hEF1a:eBFP 5ng

 

1000 nm Dox

hEF1a:rtTA 10ng

TRE:Gal4VP16 10ng

UAS:eYFP 10ng

hEF1a:mKate 10ng

hEF1a:eBFP 10ng

 

1000 nm Dox

hEF1a:rtTA 20ng

TRE:Gal4VP16 20ng

UAS:eYFP 20ng

hEF1a:mKate20ng

hEF1a:eBFP 20ng

 

1000 nm Dox

hEF1a:rtTA 50ng

TRE:Gal4VP16 50ng

UAS:eYFP 50ng

hEF1a:mKate 50ng

hEF1a:eBFP 50ng

 

1000 nm Dox

hEF1a:rtTA 100ng

TRE:Gal4VP16 100ng

UAS:eYFP 100ng

hEF1a:mKate 100ng

hEF1a:eBFP 100ng


1000 nm Dox

hEF1a:rtTA 200ng

TRE:Gal4VP16 200ng

UAS:eYFP 200ng

hEF1a:mKate 200ng

hEF1a:eBFP 200ng

 

1000 nm Dox

Red

vs.

Blue

Yellow

vs.

Blue

Image AddedImage AddedImage AddedImage AddedImage AddedImage AddedImage AddedImage Added

Co-Transfection

Efficiency

0%0%0.053%0.12%0.74%4.33%5.37%3.15%

Red

vs.

Blue

        

Yellow

vs.

Blue

        

Co-Transfection

Efficiency

        

Co-Transfection

Efficiency Average

        

Suspended Transfection

...