Versions Compared
compared with
Key
- This line was added.
- This line was removed.
- Formatting was changed.
- annealed linker oligos
- golden gate into RFP construct
- transformed golden gate product
- all linkers grew colonies except 9TCS6
- picked colonies for all except 9TCS6
- retransformed 9TCS6
- digest and gel verification
- 3TCS3, 3TCS9, 9TCSX show correct band pattern
06/24
- sent 9TCSX for sequencing
hEF1a:CD79A-XnTCSXc-Gal4VP16
| 3TCS3 | 9TCS3 | 15TCS3 | ||||||
| midi innoculated | midi verified | midi innoculated | ||||||
| 6TCS6 | 9TCS6 | 12TCS6 | ||||||
| midi verified | midi verified | midi verified | ||||||
| 3TCS9 | 6TCS9 | 9TCS9 | 12TCS9 | 15TCS9 | ||||
| midi verified | to be minied | midi innoculated | to be minied | sequencing | ||||
| 6TCS12 | 9TCS12 | 12TCS12 | ||||||
| midi verified | to be minied | to be minied | ||||||
| 3TCS15 | 9TCS15 | 15TCS15 | ||||||
| sequencing | to be minied | midi innoculated |