To transform | Plated | Mini culture | Miniprepped | Sequencing | Repeat seq | Midi culture | Frozen midipreps | Midis in seq | Completed midipreps | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CD79A-G4 |
|
| A15T9 | A3T15 | (#1, #2) - sent 8/19 | A3T15 (#2) - sent 8/17 | A9T15 (sequence after) [-80] | A9T15 |
| ||||||||||
CD79B-G4 |
|
| B3T15 B9T9 B9T15 B12T9 B15T9 |
| B3T15 (#1) - resent 8/17 B9T15 (#1) - resent 8/17 B15T15 (#2) - sent 8/16 |
| B6T9 (sequence after) [-80] B6T12 (sequence after) [-80] B9T12 [-80] B12T12 [-80] B15T3 [-80] |
| B6T9 B6T12 |
| |||||||||
Syk-tTEV | S9tTEV [-80] (digest after) S12tTEV [-80] (digest after) |
| Digest these:
| ||||||||||||||||
Syk-TEV | S6TEV - sent 8/18 | S3TEV [-80] (digest after) S9TEV [-80] (digest after) S12TEV [-80] (digest after) |
| Digest these:
| |||||||||||||||
Misc | pENTR VP16Gal4 |