...
Activating small molecule:
rtTA (Tet-ON) system:
http://www.tetsystems.com/science-technology/scientific-figures/#c22
(first diagram to the right.)
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Testing:
Primary Chassis Considered: Yeast
Middle segment can be one piece or separate, optimization?
Precise mechanism for the AND of the last part needs testing.
(NOT A) && B Gate
Degradation rate of Repressors, measured and controlled. State change time sensitive.
Look up circuitry. 6.004 ocw materials.
Propagation times and state thresholds (determined by cooperativity and binding kinetics, and etc. ).
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Motivation:
Technological Advance.
It's big.
Large system testing. (Lot's of TAL_Rs)
State based combinational system.
Memory System.
Beer.
Drugs.
Vat Production.