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Just setting a page down to see what you guys have and to share resources.
Linked list circuit
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if you add a timer to produce the advance signal, it will be a clock This uses only cas9 repressors and a small molecule signal to handle states. Key: each line is a node circuit of nodes (there isn't supposed to be a cross out, I don't know why its there) ->0'(1),0 payload,>0(3'),>0(3),->A0(0') ->1'(2),1 payload,>1(0'),>1(0),->A1(1') ->2'(3),2 payload,>2(1'),>2(1),->A2(2') ->3'(0),3 payload,>3(2'),>3(2),->A3(3') explanation of components (same comma convention): |
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initial state: \[(0),(1),(2),(3)\] present (blocking active behaviour)
all nodes are now inactive and \[(0),(1),(2),(3)\] produced |
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state change: (0') introduced
(1) stops being produced by node 0
1 becomes active
1 payload expressed
(0') produced, reinforcing 1 on state
\[(0),(2),(3),(0')\] present
state is now 1 and only one active |
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state change: (A) introduced ,now \[(0),(2),(3),(0'),(A)\] present
0,2,3 ignore it because normal suppressors active
on node 1, (1') is produced since (1) not present and (A) present ,now \[(0),(2),(3),(0'),(A),(1')\] present
(2) not produced any more, now \[(0),(3),(0'),(1')\] present
(you need to remove (A) before 2 fully active since it would cause another state transition, may also get around this by using two different (A)'s and alternating between them as well since the one that causes 1 to 2 would note cause 2 to 3, other mechanisms probably exist as well)
2 becomes active
2 payload expressed
(1') produced, enforcing 2 on state
(1) produced, deactivating 1 (the intermediate state of 1 and 2 both active automatically proceeds forwards to just 2 being active)
(0') not produced
now \[(0),(1),(3),(1')\] present
state is now 2 and only 2 active |
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future state changes: each addition of (A) will move state forward one slot ====================================================================================================== |
14 Feb Feb 9
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Applications: Time progression, biological clock. Any multi-step chemical/biological process moderated by a single organism.Beer brewing. Ethanol/Biofuel production. Drugs/Enzyme production. Chemical processes with steps that would normally interfere with one another moderated locally. Safety precaution, self-termination. Biological CS circuits. Large AND and OR Gates Add back command to create Turing Machine. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Implementation: Cas9, Guide RNA for repression. mRNA sensing repression. ====================================================================================================== |
14 Feb 12
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Updated Powerpoint with improved sprites and arrow pushing. V2 ====================================================================================================== |
14 Feb 13
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Brainstorming session. Forgot some repression arrows. Message was clear enough such that no one cared. Will fix if not lazy. -JX Cell that allows for post processing.Yeast that has the capability to creating a variety of beer. ====================================================================================================== |
14 Feb 20
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Victory. Our brave soldiers have triumphed over the forces of ??? in the battle of the first cutoff. Todo: Make a mailing list and post on wiki and igem-2014. Coordinate meeting times for preliminary experiments and circuit design. Human(e) practices? Monsanto? Control? Termination? Transfer? - JX ====================================================================================================== |
14 Feb 27
Pre-meeting Pow Wow:
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