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if you add a timer to produce the advance signal, it will be a clock This uses only cas9 repressors and a small molecule signal to handle states. Key: each line is a node circuit of nodes (there isn't supposed to be a cross out, I don't know why its there) ->0'(1),0 payload,>0(3'),>0(3),->A0(0') ->1'(2),1 payload,>1(0'),>1(0),->A1(1') ->2'(3),2 payload,>2(1'),>2(1),->A2(2') ->3'(0),3 payload,>3(2'),>3(2),->A3(3') explanation of components (same comma convention): future state changes: each addition of (A) will move state forward one slot ====================================================================================================== |
14 Feb 9
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Applications: Time progression, biological clock. Any multi-step chemical/biological process moderated by a single organism.Beer brewing. Ethanol/Biofuel production. Drugs/Enzyme production. Chemical processes with steps that would normally interfere with one another moderated locally. Safety precaution, self-termination. Biological CS circuits. Large AND and OR Gates Add back command to create Turing Machine. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ Implementation: Cas9, Guide RNA for repression. mRNA sensing repression. ====================================================================================================== |
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