...
First aliquot, 4 uL total:
- 1 uL of 5 fmol/uL tag-BFP
- 1 uL of 5 fmol/uL Hef1a promoter
- 1 uL of 10 fmol/uL Destination vector
- 1 uL of LR enzyme
Second aliquot, 4 uL total:
- 1 uL of 5 fmol/uL tag-BFP
- 1 uL of 5 fmol/uL TREt promoter
- 1 uL of 10 fmol/uL Destination vector
- 1 uL of LR enzyme
...