You are viewing an old version of this page. View the current version.

Compare with Current View Page History

Version 1 Next »

3/7/14

Made two LR reactions as practice. 

First aliquot, 4 uL total:

  • 1 uL of 5 fmol tag-BFP
  • 1 uL of 5 fmol Hef1a promoter
  • 1 uL of 10 fmol Destination vector
  • 1 uL of LR enzyme

Second aliquot, 4 uL total: 

  • 1 uL of 5 fmol tag-BFP
  • 1 uL of 5 fmol TREt promoter
  • 1 uL of 10 fmol Destination vector
  • 1 uL of LR enzyme

Stayed on the counter at room temperature overnight

  • No labels