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This protocol will create 8 60 ul aliquots of electrocompetent cells per 25 ml of outgrowth culture. I would recommend an alternative protocol if you would like to make competent cells on a large scale.

Adapted from: http://openwetware.org/wiki/Sauer:Electrocompetent_cells^{Image Removed}

Day 0

Start an overnight culture of the cells that you would like to make electrocompetent

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