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Synthetic Biology Group (Lu Lab)
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Protocols
6x Agarose gel loading dye recipe
Agarose Gel preparation
Annealing complementary Oligos
Bacterial Transformation (Version A)
Bacterial Transformation (Version B) - HIGHER EFFICIENCY?
Blue-White Screening
Copy of Making Competent Cells with TSS Buffer
Copy of Miniprep using the QIAGEN kit
Creating a SMALL batch of electrocompetent Cells
Curli Protocols
DNA sequencing protocol - Genewiz
Frozen Yeast competent cells
Gibson Assembly Master mix preparation
Guidance for Bacterial Cultures and Maintaining Plasmids
Making Competent Cells with TSS Buffer
Making Frozen stock for cells (yeast and bacteria)
Miniprep using the QIAGEN kit
phage display- amplifying T7 and fd-tet
phage display- cloning peptide libraries into T7 select system
phage display - coating ELISA plates
phage display- packaging T7 DNA
phage display- titering T7 and fd-tet
Preparing Antibiotic agar plates
Purification of small DNA fragments
Recipe for 1.1x Phusion Premix
Resuspending Oligos
Taking a Gel Image
Transforming Electrocompetent Cells
Yeast Colony PCR
Yeast Colony PCR using Kappa polymerase
Yeast Media
Yeast Transformation Protocol
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Protocols
Created by
Timothy K Lu
, last modified on
Jun 16, 2010 15:18
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