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Sucrose & xylene cyanol / bromophenol blue (6x)

  • 4g sucrose
  • 25mg bromophenol blue or xylene cyanol (0.25%)
  • dH2O to 10mL

Add appropriate amount to DNA sample, e.g. 5µl to 25µl.

Store at 4°C to avoid mould growing in the sucrose. 10mL of loading buffer will last for years.

For other agarose gel loading dyes refer here:

http://openwetware.org/wiki/Agarose_gel_loading_dye