...
(NOTE: Make sure that Buffer and Enzyme added last, enzyme after buffer)
Thermocycler:
protocol EBGG
37 37C for 5min
50X:
37 37C for 2.5min
4C for 0.5min
16C 16C for 5.5min
37C 37C for 10 min
80C 80C for 20 min
4C hold
takes 8+ hours
(check protocol by looking up the paper or other online GG protocols)