...
- Low DNA concentration (probably because incubator was not on all night)
- Sequence DNA
- Meeting with immunologist
- Pick another colony - grow it and miniprep it tomorrow
- Fusion design - design under assumption that kozak sequence is there, but sequence it to make sure
Receptor:
- Liquid culture of picked colonies didn't grow
- when we pick new colonies, put it in incubator on 2nd floor
- negative control didn't turn blue
- plates are not the right kind
- mutation in lacZ
- likely that reaction happened successfully
- to check: miniprep dna from colonies, digest it and run a gel to figure out whether or not the gblocks are there
- sequence dna and look for mutations (frameshifts, stop codon, L sites)