| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | In close contact | ||
Jeremy Gam | Questions | Had first meeting |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Journal Club
- Finish presentation
- Practice presentation
- Run through NEGEM Presentation
miRNA Sensors (Theory)
- Put in order for miRNA and other parts
- Write out detailed plan for cloning and Gibson/Gateway reactions
What How does repressor affect the circuit? - Determine final output of circuit
miRNA Sensors (Lab Work)
- Understand why we're doing
- Why we're doing it
- What we need
- Determine repressor
- Determine output
- Perform necessary lab work
- any of these reactions
- Transform MAV1212 into ccdB resistance cells
- Grow at low temp w/ x-gal, shielded from light
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique