| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Meeting before NEGEM | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Create a list/diagram of plasmids based on morning meeting
- Use AND logic --> each sensor on it's own plasmid?
- Create a workflow of plasmids to build (in parallel)
- Update the parts list on the wiki
miRNA Sensors (Theory)
- Prepare to meet with Jake Beal
miRNA Sensors (Lab Work)
- Transform Re-transform MAV1212
- Re-transform MAV1212-Hef1a-mKate2/MAV1212eBFP2
- Mini prep MAV1212-Hef1a-EBFP2
- Put together LacZ PCR product (check protocol)
- eBFP2
- Gateway MAV1212-Hef1a-mKate2
- Gateway MAV1212-Hef1a-L7ae
Cell Models
- Figure out what's going on