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Word | Meaning |
---|---|
in planning | being designed in geneious |
designed | plasmid design completed in geneious |
in lab | someone else in the lab has it |
ordered | |
in construction | |
constructed | |
in storage | we have it in our fridge/freezer, etc |
in culture | received in E. coli culture, has not been mini-prepped yet |
NAME | Dependency | GROUP | STATUS | TYPE | LOCATION | INITIALS | CONSTRUCTED ON | OBTAINED FROM | PRIORITY | NOTES | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
pEXPR hEF1a: Gmab Heavy | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: Gmab Light | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: CD79A | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: CD79B | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: CD79A-TCS-Gal4VP16 | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: CD79B-TCS-Gal4VP16 | Antibodies | Planned | expression | |||||||||||||||||
pEXPR hEF1a: Lyn | Antibodies | Planned | expression | |||||||||||||||||
pEXPR PTet: Lyn-mKate | Antibodies | Planned | expression | |||||||||||||||||
pEXPR PTet: Syk-eYFP | Antibodies | Planned | expression | |||||||||||||||||
pEXPR PTet: Syk-TEV | Antibodies | Planned | expression | |||||||||||||||||
pEXPR PTet: eBFP | Antibodies | In Freezer | expression | |||||||||||||||||
pEXPR Gal4/UAS: mKate | Antibodies | In Freezer | expression | iGEM -20 | CR | N/A | Velia | High | ||||||||||||
pEXPR hEF1a: rtTA | Antibodies | In Freezer | expression | iGEM -20 | CR | N/A | Velia | High | ||||||||||||
pEXPR Gal4UAS:eYFP | Antibodies | in someone else's freezer | expression | |||||||||||||||||
pEXPR Gal4UAS:mKate | Antibodies | in someone else's freezer | expression | |||||||||||||||||
pEXPR Tre-TEVp | Antibodies | in someone else's freezer | expression | |||||||||||||||||
pENTR Gmab Heavy | Antibodies | Ordered | expression | |||||||||||||||||
pENTR Gmab Light | Antibodies | Ordered | expression | |||||||||||||||||
pENTR CD79A | Antibodies | in stock | expression | |||||||||||||||||
pENTR CD79B | Antibodies | in stock | expression | |||||||||||||||||
pENTR CD79A-TCS-Gal4VP16 | Antibodies | Planned | expression | |||||||||||||||||
pENTR CD79B-TCS-Gal4VP16 | Antibodies | Planned | expression | |||||||||||||||||
pENTR Lyn | Antibodies | In culture | expression | |||||||||||||||||
pENTR Lyn-mKate | Antibodies | Planned | expression | |||||||||||||||||
pENTR Syk-eYFP | Antibodies | Planned | expression | |||||||||||||||||
pENTR Syk-TEV | Antibodies | Planned | expression | |||||||||||||||||
pENTR eBFP | Antibodies | ? | expression | |||||||||||||||||
pENTR mKate | Antibodies | ? | expression | iGEM -20 | CR | N/A | Velia | High | ||||||||||||
pENTR rtTA | Antibodies | ? | expression | iGEM -20 | CR | N/A | Velia | High | ||||||||||||
pENTR eYFP | Antibodies | ? | expression | |||||||||||||||||
pENTR mKate | Antibodies | ? | expression | |||||||||||||||||
gBlock mIgM Gmab Heavy | ||||||||||||||||||||
pENTR Tre-TEVp | Treatment | in the lab already | none | Antibodies | ordered | gBlock | gBlock mIgM Gmab Light | none | Antibodies | ordered | ||||||||||
| Treatment | planned | gBlock | |||||||||||||||||
| pENTR Syk (pDONR223) | none | Antibodies | in culture | entry | Treatment | to be ordered | pENTR Lyn (pDONR223) | none | Antibodies | in culture | entry | ||||||||
| pENTR CD79A (pDONR223) | none | Antibodies | in storage | Treatment | in the lab already | entry | pENTR CD79B (pDONR223) | none | Antibodies | in storage | |||||||||
| Treatment | in the lab already | entry | |||||||||||||||||
pEXPR U6:mKate-miRNA(intronic) | Treatment | planned | ||||||||||||||||||
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) | Treatment | planned | ||||||||||||||||||
Experiment 1 | ||||||||||||||||||||
| Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Kyle | not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough | ||||||||||||
| Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Brandon | will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock | ||||||||||||
| Receptor | in lab | plasmid | will be used to make LilrB2 expression vector | ||||||||||||||||
| Receptor | in lab | plasmid | will be used to make LilrB2 expression vector | ||||||||||||||||
| Receptor | in construction | plasmid | waiting on entry vector | ||||||||||||||||
| Receptor | in construction | plasmid | ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA | ||||||||||||||||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||||||||||||
| Receptor | in construction | waiting on entry vector | |||||||||||||||||
| Receptor | in construction | failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA | |||||||||||||||||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||||||||||||
| Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||||||||||||
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