| NAME | Dependency | GROUP | STATUS | TYPE | LOCATION | INITIALS | CONSTRUCTED ON | OBTAINED FROM | PRIORITY | NOTES |
|---|---|---|---|---|---|---|---|---|---|---|
| pEXPR hEF1a: Gmab Heavy | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: Gmab Light | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79A | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79B+stop | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79A-TCS-Gal4VP16 | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: CD79B-TCS-Gal4VP16 | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR hEF1a: Lyn+stop | pENTR hEF1a | Antibodies | designed | expression | ||||||
| pEXPR TRE: Lyn-mKate | pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR TRE: Syk-eYFP | pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR PTet: Syk-TEVp | pENTR Syk-TEVp pENTR PTet | Antibodies | designed | expression | ||||||
| pEXPR TRE: eBFP | pENTR eBFP | Antibodies | in storage? | expression | ||||||
| pEXPR Gal4/UAS: mKate | - | Antibodies | in storage | expression | iGEM -20 | CR | N/A | Velia | High | |
| pEXPR hEF1a: rtTA | pENTR rtTA | Antibodies | in storage | expression | iGEM -20 | CR | N/A | Velia | High | |
| pEXPR Gal4UAS:eYFP | - | Antibodies | in storage | expression | iGEM -20 | CR | N/A | Velia | High | |
| pEXPR Tre-TEVp | - | Antibodies | in lab | expression | ||||||
| pENTR Gmab Heavy | gBlock mIgM Gmab Heavy | Antibodies | Ordered | entry | IDT | |||||
| pENTR Gmab Light | gBlock mIgM Gmab Light | Antibodies | Ordered | entry | IDT | |||||
| pENTR CD79A (pDONR223) | - | Antibodies | in storage | entry | iGEM -20 | DNASU | ||||
| pENTR CD79A+stop | pENTR CD79A (pDONR223) | Antibodies | in construction | entry | ||||||
| pENTR CD79B (pDONR223) | - | Antibodies | in storage | entry | iGEM -20 | DNASU | ||||
| pENTR CD79B+stop | pENTR CD79B (pDONR223) | Antibodies | in construction | entry | ||||||
| pENTR CD79A-TCS-Gal4VP16 | pENTR CD79A (pDONR223) pEXPR hEF1a-FGFR-mKate-TCS(L)-G4 | Antibodies | in construction | entry | ||||||
| pENTR CD79B-TCS-Gal4VP16 | pENTR CD79B | Antibodies | in construction | entry | ||||||
| pENTR Lyn (pDONR223) | - | Antibodies | in storage | entry | iGEM -20 | addgene | ||||
| pENTR Lyn+stop | pENTR Lyn (pDONR223) | Antibodies | in construction | entry | ||||||
| pENTR Lyn-mKate | pENTR Lyn pENTR mKate | Antibodies | in construction | entry | ||||||
| pENTR Syk (pDONR223) | - | Antibodies | in storage | entry | iGEM -20 | addgene | ||||
| pENTR Syk+stop | pENTR Syk (pDONR223) | Antibodies | in construction | entry | ||||||
| pENTR Syk-eYFP | pENTR Syk pENTR eYFP | Antibodies | in construction | entry | ||||||
| pENTR Syk-TEVp | pENTR Syk pEXPR Tre-TEVp | Antibodies | in construction | entry | ||||||
| pENTR eBFP | - | Antibodies | in storage? | entry | ||||||
| pENTR L1_mKate_L2 | - | Antibodies | in storage | entry | ||||||
| pENTR rtTA | - | Antibodies | in storage | entry | ||||||
| pENTR_L1_EYFP_L2 | - | Antibodies | in storage | entry | ||||||
| pEXPR hEF1a-FGFR-mKate-TCS(L)-G4 | - | Antibodies | in storage | entry | iGEM -20 | KB | N/A | Velia | High | |
| gBlock mIgM Gmab Heavy | - | Antibodies | ordered | gBlock | IDT | |||||
| gBlock mIgM Gmab Light | - | Antibodies | ordered | gBlock | IDT | |||||
| pEXPR Tre: BACE2-YFP |
| Treatment | in construction | expression | ||||||
pENTR L1--BACE2-eYFP--L2 |
| Treatment | in construction | entry | ||||||
Q1-BACE2-Q2 | BACE2 | Treatment | in construction | PCR product | ||||||
| BACE2 | n/a | Treatment | in the lab | expression | ||||||
Q2-eYFP-QX | pEXPR eYFP | Treatment | in the lab | PCR product | ||||||
| pEXPR eYFP | n/a | Treatment | in the lab | expression | ||||||
pENTR Tre | n/a | Treatment | in the lab | entry | ||||||
pDEST R4--R2 | n/a | Treatment | in the lab | destination | ||||||
| pEXPR Tre: BACE1-YFP |
| Treatment | planned | expression | ||||||
pENTR L1--BACE1-eYFP--L2 |
| Treatment | planned | entry | ||||||
Q1-BACE1-Q2 | BACE1 | Treatment | planned | PCR product | ||||||
| BACE1 | n/a | Treatment | in the lab | expression | ||||||
Q2-eYFP-QX | pEXPR eYFP | Treatment | in the lab | PCR product | ||||||
pENTR Tre | n/a | Treatment | in the lab | entry | ||||||
pDEST R4--R2 | n/a | Treatment | in the lab | destination | ||||||
| pEXPR U6:mKate-miRNA(intronic) | Treatment | in planning | expression | |||||||
| pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) | Treatment | in planning | expression | |||||||
ggDONR L1_LacZ_L2 | Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Kyle |
| ||
ggDONR L1_LacZ_L2 (New) | Receptor | in storage | plasmid | +4 in iGEM room | LA | - | Brandon | |||
pENTR L4_Hef1a_R1 | Receptor | in lab | plasmid | |||||||
pDEST | Receptor | in lab | plasmid | |||||||
pEXPR Hef1a:LilrB2 | pENTR L1_LilrB2_L2 pENTR L4_Hef1a_R1 | Receptor | waiting on part | plasmid | waiting on entry vector | |||||
pENTR L1-LilrB2-L2 | LilrB2 gBlock | Receptor | to be verified | -4 in iGEM room | SS | - | ||||
LilrB2 gBlock | Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
pEXPR Hef1a:PirB | pENTR L1_PirB_L2 pENTR L4_Hef1a_R1 | Receptor | Waiting on part | expression | waiting on entry vector | |||||
pENTR L1_PirB_L2 | PirB gBlock C terminus PirB gBlock N terminus | Receptor | to be verified | entry | ||||||
PirB gBlock C terminus | Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
PirB gBlock N terminus | Receptor | in storage | gBlock | -20 in iGEM room | LA | - | IDT | used in Golden Gate | ||
| PDONR MAV1212 | MAV1212 | miRNA sensing | in storage | donor | -20 in iGEM room | RR | N/A | Jeremy Gam | High | Original Multi Assembly Vector, currently under cloning |
| pENTR L1_hEF1A_L2 | - | miRNA sensing | in lab? | entry | ? | RR | N/A | N/A | High | Stock in iGEM room may exist, but the one I found was empty |
| pENTR R1_l7ae_L4 | - | miRNA sensing | in storage | entry | -20 in iGEM room | RR | N/A | N/A | High | |
| pEXPR hEF1A:l7ae | MAV1212 | miRNA sensing | in construction | expression | N/A | RR | N/A | N/A | High | |
| pENTR R1_mKate_L4 | - | miRNA sensing | in storage | entry | -20 in iGEM room | RR | N/A | N/A | High | |
| MAV1212 hEF1A:eBFP2 | MAV1212 | miRNA sensing | in storage | positive control plasmid | -20 in iGEM room | RR | N/A | Jeremy Gam | Medium | |
| PENTR R1_eYFP_L4 | - | miRNA sensing | in storage | entry | -20 in iGEM room | RR | N/A | N/A | High | |
| pEXPR hEF1A:mKate2 | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:144 | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:181c | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:mKate2:144:181c | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:30d | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:let7f | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:125b | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:146a | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| pEXPR hEF1A:eYFP:30d:let7f:125b:146a | MAV1212 | miRNA sensing | in planning | expression | N/A | RR | N/A | N/A | High | |
| Q1_144_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |
| Q1_181c_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |
| Q1_30d_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |
| Q1_let7f_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |
| Q1_125b_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |
| Q1_146a_Q2 | miRNA target site | miRNA sensing | in lab | target site | Jeremy Gam's Lab | RR | N/A | IDT | High | |