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Put recently arrived primers in solution to 100uM using TE buffer (added and vortexed).
PCR
Ran PCRs for the following samples:
Product | Gene | Starting Plasmid | Forward Primer | Reverse Primer | Tm hi/lo (anneal) |
|---|---|---|---|---|---|
| CD79A | CD79A | pENTR CD79A (pDONR223) | iGEM KRB 003 F | iGEM KRB 004 R | 62/61 (61) |
| CD79A-TCS | CD79A | pENTR CD79A (pDONR223) | iGEM KRB 003 F | iGEM KRB 005 R | 62/61 (61) |
| mKate | mKate | pENTR_L1_mKate_L2 | iGEM KRB 006 F | iGEM KRB 007 R | 58/61 (58) |
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