DateDescription
2014 June 22PCRs for CD79A+stop, CD79A-TCS, linker+mKate
2014 June 24DpnI digest for mKate and eYFP
2014 June 26

DpnI digest for TEVp

Transformations of Golden Gates (CD79A+stop, CD79A-TCS-Gal4VP16, Syk+stop)

Golden Gates (Lyn+stop, Lyn-mKate, Syk-eYFP, Lyn-TEV, Syk-TEV)

2014 June 27

Picking colonies (CD79A+stop, CD79A-TCS-Gal4VP16, Syk+stop)

pUC19 competence

2014 June 29Picking colonies (Lyn+stop, Lyn-mKate, Lyn-TEVp, Syk-eYFP, Syk-TEVp)
2014 June 30 

 

 

 

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