...
Lane 2: CD79A + stop codon (724bp)
Lane 3: CD79A + TCS (718bp)
Lane 4: linker + mKate (768bp)
When loading wells with PCR products, used Parafilm to mix 1uL of 6X Orange dye with 5uL of sample and loaded 5uL of this mixture in a lane. Ran gel at 120V for 30 minutes.
