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People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact

 

Long Term Goals

miRNA Profile

  •  Read literature
  •  Choose profile

Building the Cell Detector Circuit

  •  Build mathematical model
  •  Make test bed
    •  Controlled miRNA expression
    •  Constructed miRNA sensors

Mini experiments

    •  Single miRNA detector circuit

Alzheimer's Model

  •  Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

  •  Present about cell models
  •  COMPLETE PARTS LIST ON WIKI

miRNA Sensors (Theory)

  •  Prep experiments
  •  Contact Jeremy about multiple miRNA target sites chained together

miRNA Sensors (Lab Work)

  •  Pick colonies for MAV1212
  •  Miniprep MAV1212
  •  Keep MAV1212-Hef1a-eBFP2 (for use WITHOUT target sites)
  •  Gateway MAV1212-Hef1a-L7ae (for high sensor)
  •  Gateway MAV1212_Hef1a-eYFP (for use WITH target sites)