| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Present about cell models
- COMPLETE PARTS LIST ON WIKI
miRNA Sensors (Theory)
- Prep experiments
- Contact Jeremy about multiple miRNA target sites chained together
miRNA Sensors (Lab Work)
- Pick colonies for MAV1212
- Miniprep MAV1212
- Keep MAV1212-Hef1a-eBFP2 (for use WITHOUT target sites)
- Gateway MAV1212-Hef1a-L7ae (for high sensor)
- Gateway MAV1212_Hef1a-eYFP (for use WITH target sites)
- Transform Hef1a, MAV1212 ( ? )